Authors: Xet-Mull A.-M. 1; M. Kubín 2,3; V. Príkazský 2; E. H. Harris 1; L. W. Riley 1 Authors‘ workplace: School of Public Health, University of California in Berkeley, CA, USA 2 Státní zdravotní ústav, Praha 3 Hygienická stanice hlavního města Prahy Published in: Epidemiol. Mikrobiol. Imunol. , 1999, č. 2, s. 82-86 Category:
In a pilot study Double Repetitive Element-Polymerase Chain Reaction 20 clinical is olates ofMycobacterium tuberculosis from Guatemala and 49 strains from Prague were typed. This techniqueis based on direct evidence of repetitive elements IS6110 or PGRS and does not require DNApurification, digestion by endonuclease nor Southern blot hybridization. Preliminary examinationof Guatemalian strains revealed a striking identity or similarity of DRE-PCR profiles while thePrague strains were characterized by conspicuous polymorphism. The Prague strains were exami-ned in a total number of 13 series of electrophoreograms and subsequently subjected to automatedanalysis with GelCompar software. The DRE-PCR method is suitable for screening of a majornumber of clinical isolates of M. tuberculosis in laboratories equipped with a minimum of technicalfacilities for the PCR method, furthermore it requires facilities for synthesis of the necessaryprimers and at least basic knowledge of molecular biology.
Key words: DRE-PCR – Mycobacterium tuberculosis.
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