Studying the link between physiological performance of Crotalaria ochroleuca and the distribution of Ca, P, K and S in seeds with X-ray fluorescence

Autoři: Mayara Fávero Cotrim aff001;  Josué Bispo da Silva aff002;  Flávia Mendes dos Santos Lourenço aff003;  Anielli Verzotto Teixeira aff001;  Ricardo Gava aff001;  Charline Zaratin Alves aff001;  Ana Carina da Silva Candido aff001;  Cid Naudi Silva Campos aff001;  Márcio Dias Pereira aff004;  Salvador Barros Torres aff005;  Gianluigi Bacchetta aff006;  Paulo Eduardo Teodoro aff001
Působiště autorů: Federal University of Mato Grosso do Sul—UFMS, Chapadão do Sul, MS, Brazil aff001;  Federal University of Mato Grosso do Sul—UFMS, Três Lagoas, MS, Brazil aff002;  State University of Sao Paulo–UNESP, Ilha Solteira, SP, Brazil aff003;  Federal University of Rio Grande do Norte–UFRN, Natal, RN, Brazil aff004;  Federal University of the Semi-arid–UFERSA, Mossoró, RN, Brazil aff005;  Universitá Degli Studi Di Cagliari, Cagliari—CA, Italy aff006
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222987


This study describes the use of X-ray fluorescence spectroscopy in Crotalaria ochroleuca seed technology. This work evaluated X-ray fluorescence techniques to estimate the physiological performance of different C. ochroleuca seed coat colours based on the concentration and distribution of Ca, P, K, and S in seed structures. The treatments consisted of seeds separated by coat colours (yellow, green, and red) and a control treatment (colour mix according to their natural occurrence in commercial lots), and was carried out in a completely randomized design, with four replications. The physiological performance was evaluated by analyzing the water content, germination, first germination count, germination speed index, electrical conductivity, seedling emergence, and seedling length and dry mass. X-ray fluorescence spectroscopy techniques were carried out with quantitative analyses (Ca, P, K, and S concentration in the seed coat and the whole seed) and qualitative analyses (macronutrient mapping). The EDXRF and μ-XRF techniques are efficient and promising to differentiate the physiological performance of C. ochroleuca seeds, based on the concentration and distribution of Ca, P, K, and S in different structures. Ca is predominant in the seed coat, and K, S, and P are found throughout the embryonic axis. Seeds of yellow and green coats have higher nutrients concentration and distribution in the embryonic axis, revealing high germinative capacity and physiological performance. Seeds of red coat have higher nutrients concentration in the seed coat and lower assimilation, showing less vigour, which interferes directly in the quality of commercial lots.

Klíčová slova:

Electric conductivity – Embryos – Seed germination – Seedlings – Seeds – Seed coat – Radicle – Hypocotyl


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