#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Protective mechanism of 1-methylhydantoin against lung injury induced by paraquat poisoning


Autoři: Bo Liu aff001;  Annan Chen aff001;  Jinyi Lan aff001;  Lei Ren aff001;  Yifan Wei aff002;  Lina Gao aff003
Působiště autorů: The 3 Clinical Department of China Medical University, Shenyang, Liaoning, China aff001;  School of Public Health, China Medical University, Shenyang, Liaoning, China aff002;  School of Forensic Medicine, China Medical University, Shenyang, Liaoning,China aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0222521

Souhrn

Paraquat (PQ), one of the most widely used herbicides worldwide, causes severe toxic effects in humans and animals. 1-methylhydantoin (MH) is an active ingredient of Ranae Oviductus, which has broad pharmacological activities, e.g., eliminating reactive oxygen species and inhibiting inflammation. This study investigated the effects of MH on lung injury induced by PQ. A PQ poisoning model was established by intragastric infusion of PQ (25 mg/kg), and the control group was simultaneously gavaged with the same dose of saline. The MH group was intraperitoneally injected with 100 mg/kg once per day after intragastric infusion of PQ (25 mg/kg) for five consecutive days. All animals were sacrificed on the sixth day, and the lung tissues were dissected for metabolomics analysis. The lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD) activity, TNF-α and malondialdehyde (MDA) content were determined according to the instructions of the detection kit. Compared with that in the control group, the content of LDH, TNF-α and MDA in the lung tissue of the PQ group was significantly higher, and the activity of SOD in the lung tissue was significantly lower (all p<0.05). Compared with that in the control group, the content of LDH, TNF-α and MDA in the MH group was significantly higher, and the activity of SOD was significantly lower (all p<0.05). However, the differences in SOD activity, LDH activity between the PQ and MH groups were not statistically significant (all p > 0.05). There were significant differences in MDA and TNF-α content between the PQ group and MH group (all p<0.05). MH decreased the production of malondialdehyde and TNF-α to protect against the lung injury caused by PQ poisoning, but it had no significant effect on the activity of LDH and SOD. There were significant differences in metabolomics between the MH group and the PQ poisoning group, primarily in bile acid biosynthesis and metabolism of cholesterol, nicotinate, nicotinamide, alanine, aspartate, glutamate, glycine, threonine, serine, phenylalanine and histidine. Therefore, this study highlights that MH has non-invasive mechanisms and may be a promising tool to treat lung injury induced by PQ poisoning.

Klíčová slova:

Drug metabolism – Inflammation – Metabolic pathways – Metabolites – Poisoning – Superoxide dismutase – Nicotine – Protein metabolism


Zdroje

1. Blanco-Ayala T, Andérica-Romero AC, Pedraza-Chaverri J. New insights into antioxidant strategies against paraquat toxicity. Free Radic Res, 2014;48(6):623–640. doi: 10.3109/10715762.2014.899694 24593876

2. Sun B, He Y. Paraquat poisoning mechanism and its clinical treatment progress. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue, 2017;29(11):1043–1046. doi: 10.3760/cma.j.issn.2095-4352.2017.11.018 29151425

3. Awadalla EA. Efficacy of vitamin C against liver and kidney damage induced by paraquat toxicity. Exp Toxicol Pathol, 2012;64(5):431–434. doi: 10.1016/j.etp.2010.10.009 21130632

4. Rodrigues da Silva M, Schapochnik A, Peres Leal M, Esteves J, Bichels Hebeda C, Sandri S, et al.Beneficial effects of ascorbic acid to treat lung fibrosis induced by paraquat.Plos One, 2018;13(11):e0205535.

5. Gales L, Amorim R, Afonso CM, Carvalho F, Dinis-Oliveira RJ.Decreasing the toxicity of paraquat through the complexation with sodium salicylate: Stoichiometric analysis.Toxicology,2015;336:96–8. doi: 10.1016/j.tox.2015.08.005 26298007

6. Amberg A, Riefke B, Schlotterbeck G, Ross A, Senn H, Dieterle F, et al. NMR and MS methods for metabolomics. Methods Mol Biol, 2017;1641:229–258. doi: 10.1007/978-1-4939-7172-5_13 28748468

7. Kusonmano K, Vongsangnak W, Chumnanpuen P. Informatics for Metabolomics. Adv Exp Med Biol,2016;939:91–115. doi: 10.1007/978-981-10-1503-8_5 27807745

8. Ling XM, Zhang XH, Tan Y, Yang JJ, Ji B, Wu XR, et al.Protective effects of Oviductus Ranae-containing serum on oxidative stress-induced apoptosis in rat ovarian granulosa cells.J Ethnopharmacol., 2017; 208:138–148.

9. Zhang Y, Liu Y, Zhu K, Dong Y, Cui H, Mao L, et al. Acute Toxicity, Antioxidant, and Antifatigue Activities of Protein-Rich Extract from Oviductus ranae.Oxid Med Cell Longev.2018:9021371. doi: 10.1155/2018/9021371 2018; eCollection 2018. 29991975

10. Hang H. Study on the synthesis of 1-methylhydantoin. Speciality Petrochemicals 2016;33(3):78–81.

11. Wang Y, Yang Y, Li Y, Li G.Synthesis of 1-Methylhydantoin and Its Crystal Structure.Journal of Jilin University (SCIENCE EDITION),2007;45(4):657–660.

12. Li W, Wang Q, Zhou X, Liu L, Li X, Wang S. Research of SW-480 cells by 1-methylhydantoin. China Journal of Modern Medicine,2014;24(9):17–20.

13. Han D, Dong X, Qiu Z. Antiasthmatic effect of 1-methylhydantoin on rat asthma model and its mechanism. Journal of Jilin University (Medicine Edition), 2014;40(3):543–548.

14. You J, Zhang R, Wang C. Effects of 1- methylhydantoin on behavior changes in depressive rats and its possible mechanisms.Chin Pharmacol Bull, 2013;29 (8): 1104–1108.

15. Blanco-Ayala T, Andérica-Romero AC, Pedraza-Chaverri J.New insights into antioxidant strategies against paraquat toxicity. Free Radic Res, 2014; 48(6): 623–40. doi: 10.3109/10715762.2014.899694 24593876

16. Chang TH, Tung KH, Gu PW, Yen TH, Cheng CM. Rapid simultaneous determination of paraquat and creatinine in human serum using a piece of paper. Micromachines (Basel), 2018:9(11). doi: 10.3390/mi9110586 30424506

17. Ienaga K, Park CH, Yokozawa T. Protective effect of an intrinsic antioxidant, HMH (5-hydroxy-1-methylhydantoin;NZ-419), against cellular damage of kidney tubules. Exp Toxicol Pathol, 2013;65(5):559–566. doi: 10.1016/j.etp.2012.05.001 22749566

18. Ienaga K, Yokozawa T.Treatment with NZ-419 (5-Hydroxy-1-methylimidazoline-2,4-dione), a novel intrinsic antioxidant, against the progression of chronic kidney disease at stages 3 and 4 in rats.Biol Pharm Bull., 2010; 33(5):809–15. doi: 10.1248/bpb.33.809 20460759

19. Hasegawa G, Nakano K, Ienaga K. Serum accumulation of a creatinine oxidative metabolite (NZ-419: 5-hydroxy-1- methylhydatoin) as an intrinsic antioxidant in diabetic patients with or without chronic kidney disease.Clin Nephrol.,2011; 76(4):284–289. doi: 10.5414/cn107025 21955863

20. Gao L, Yang S, Liu J, Liu L. Preventive effects of 5-hydroxy-1-methylhydantoin on paraquat-induced nephrotoxicity in rat. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue, 2015; 27(4):246–249. doi: 10.3760/cma.j.issn.2095-4352.2015.04.004 25891451

21. Gao L, Yuan H, Cao Z, Xu E, Liu J. Exploration about the protection mechanism of 5-hydroxy-1-methylhydantoin on paraquat poisoning model.Zhonghua Wei Zhong Bing Ji Jiu Yi Xue,2018; 30(12):1184–1189. doi: 10.3760/cma.j.issn.2095-4352.2018.012.016 30592955

22. Nakamura K, Ienaga K, Yokozawa T, Fujitsuka N, Oura H.Production of methylguanidine from creatinine via creatol by active oxygen species: analyses of the catabolism in vitro.Nephron., 1991; 58(1):42–6. doi: 10.1159/000186376 1649975

23. Ienaga K, Nakamura K, Naka F, Goto T. The metabolism of 1-methylhydantoin via 5-hydroxy-1-methylhydantoin in mammals. Biochim Biophys Acta. 1988; 967(3):441–3. doi: 10.1016/0304-4165(88)90108-0 3196760

24. Donnahoo KK, Meng X, Ayala A, Cain MP, Harken AH, Meldrum DR. Early kidney TNF‐α expression mediates neutrophil infiltration and injury after renal ischemia‐reperfusion. American Journal of Physiology‐Regulatory, Integrative and Comparative Physiology,1999;277: R922–929.

25. Castello PR, Drechsel DA, Patel M. Mitochondria are a major source of paraquatinduced reactive oxygen species production in the brain. J. Biol. Chem., 2007;282, 14186–14193.

26. Bonneh-Barkay D, Reaney SH, Langston WJ, Di Monte DA. Redox cycling of the herbicide paraquat in microglial cultures. Brain Res. Mol. Brain Res.,2005; 134, 52–56. doi: 10.1016/j.molbrainres.2004.11.005 15790529

27. Roede JR, Uppal K, Park Y, Tran V, Jones DP.Transcriptome–metabolome wide association study (TMWAS) of maneb and paraquat neurotoxicity reveals network level interactions in toxicologic mechanism.Toxicology Reports, 2014;1, 435–444. doi: 10.1016/j.toxrep.2014.07.006 27722094

28. Broadhurst DI, Kell DB. Statistical strategies for avoiding false discoveries in metabolomics and related experiments. Metabolomics, 2006; 2, 171–196.

29. Gao Y, Li W, Chen J, Wang X, Lv Y, Huang Y, et al. Pharmaco metabolomic prediction of individual difffferences of gastrointestinal toxicity complicating myelosuppression in rats induced by irinotecan. Acta Pharm. Sin. B, 2019;9, 157–166. doi: 10.1016/j.apsb.2018.09.006 30766787

30. Hantson P, Weynand B, Doyle I, Bernand A, Hermans C. Pneumoproteins as markers of paraquat lung injury: a clinical case. J Forensic Legal Med,2008;15(1):48–52.

31. Liu G, Song D, Jiang Y, Zhu L, Ge Y. Expression of heme oxygenase-1 in lung tissue of paraquat poisoned mice and its significance.Chin Crit Care Med,2015;27(4):280–284.

32. Jian XD, Li M, Zhang YJ, Ruan Y, Guo G, Sui H. Role of growth factors in acute lung injury induced by paraquat in a rat model. Hum Exp Toxicol, 2011; 30(6):460–469. doi: 10.1177/0960327110372648 20498031

33. Yongsheng Wang, Hui Zhang, Zhe Lin.Study on Determination of 1-MethylHydantoin inOviductus ranaeby RP-HPLC Assay. Chin Pharm J,2008;43(2):146–148.

34. Jingjing Yang, Xiaomei Ling, Xuhui Zhang. Determination of anti-aging components of OViductus Ranae extracts in rat serum by HPLC. Journal of Guangdong Pharmaceutical University,2018;34(4):417–421.

35. Pakula MM, Maier TJ, Vorup-Jcnsen T. Insilght on the impacts of free amino acids and their metabolites on the immune system from a perspective of inborn errors of amino acid metabolism.Expert Opin Ther Targets, 2017;21(6):611–626. doi: 10.1080/14728222.2017.1323879 28441889


Článek vyšel v časopise

PLOS One


2019 Číslo 9
Nejčtenější tento týden
Nejčtenější v tomto čísle
Kurzy

Zvyšte si kvalifikaci online z pohodlí domova

KOST
Koncepce osteologické péče pro gynekology a praktické lékaře
nový kurz
Autoři: MUDr. František Šenk

Sekvenční léčba schizofrenie
Autoři: MUDr. Jana Hořínková

Hypertenze a hypercholesterolémie – synergický efekt léčby
Autoři: prof. MUDr. Hana Rosolová, DrSc.

Svět praktické medicíny 5/2023 (znalostní test z časopisu)

Imunopatologie? … a co my s tím???
Autoři: doc. MUDr. Helena Lahoda Brodská, Ph.D.

Všechny kurzy
Kurzy Podcasty Doporučená témata Časopisy
Přihlášení
Zapomenuté heslo

Zadejte e-mailovou adresu, se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se

#ADS_BOTTOM_SCRIPTS#