Protective effect of lactobacillus plantarum on alcoholic liver injury and regulating of keap-Nrf2-ARE signaling pathway in zebrafish larvae

Autoři: Yaping Liu aff001;  Xiaoqian Liu aff003;  Ying Wang aff001;  Cao Yi aff001;  Jiahui Tian aff001;  Kechun Liu aff001;  Jie Chu aff001
Působiště autorů: Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong, China aff001;  School of Medical Instrument and Food Engineering, University of Shanhai for Science and Technology, Shanghai, China aff002;  Department of General Practice of Shandong Provincial Qianfoshan Hospital, Ji’nan, Shandong, China aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222339


This research investigated the protective effect of lactobacillus plantarum against alcohol-induced liver injury and the regulatory mechanism of Keap-Nrf2-ARE signal pathway in zebrafish. Firstly, a zebrafish alcoholic liver injury model was established using1.0mM of ethanol concentration, then two forms of lactobacillus plantarum treatment were designed to perform repair, including a lactobacillus plantarum thallus suspension (LPS) and a lactobacillus plantarum thallus breaking solution (LPBS). After 24h of alcohol injury, lactobacillus plantarum concentrations of 0, 1.0×105, 1.0×106, 1.0×107 and 1.5×107 cfu/mL were added to protect zebrafish larvae. Then with the treatment of lactobacillus plantarum after 48h, activities of alanine transaminase (ALT), aspartate transaminase (AST), superoxide dismutase (SOD) and malondialdehyde (MDA) in zebrafish tissue homogenate were respectively determined. Keap-Nrf2-ARE signal pathway related gene expression conditions were also analyzed, including nuclear factor (erythroid-derived 2)-like 2(Nrf2), Kelch like ECH associated protein 1(Keap1), catalase(CAT), hemooxygenase1(HO1) and Glutathione S-Transferase Kappa 1(gstk1). Results showed that: in comparison with the control group, the LPBS with dosage of 1.0×107 cfu/mL remarkably improved the activities of SOD, CAT, HO1and gstk1 in zebrafish larvae liver (P<0.05), resulting in significant increase of the protein expression level of Nrf2 (225.78%) and suppression of Keap1 gene expression (73.67%)(P<0.01). As confirmed by the results, lactobacillus plantarum activated the Keap-Nrf2-ARE signal pathway from the level of transcription, the up-regulation of the expression quantity of Nrf2 protected the organism from oxidative stress and maximally reduced liver injury.

Klíčová slova:

Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Zebrafish – Animal models – Biology and life sciences – Organisms – Eukaryota – Animals – Vertebrates – Fish – Osteichthyes – Bacteria – Gut bacteria – Lactobacillus – Developmental biology – Life cycles – Larvae – Embryology – Embryos – Cell biology – Oxidative stress – Psychology – Addiction – Addicts – Alcoholics – Physical sciences – Chemistry – Chemical compounds – Organic compounds – Alcohols – Ethanol – Organic chemistry – Social sciences


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