Phylogenic classification and virulence genes profiles of uropathogenic E. coli and diarrhegenic E. coli  strains isolated from community acquired infections


Autoři: Rasha M. Khairy aff001;  Ebtisam S. Mohamed aff001;  Hend M. Abdel Ghany aff002;  Soha S. Abdelrahim aff001
Působiště autorů: Department of Microbiology and Immunology, Faculty of Medicine, Minia University, Minia, Egypt aff001;  Department of Biochemistry, Faculty of Medicine, Minia University, Minia, Egypt aff002;  Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222441

Souhrn

The emergence of E.coli strains displaying patterns of virulence genes from different pathotypes shows that the current classification of E.coli pathotypes may be not enough, the study aimed to compare the phylogenetic groups and urovirulence genes of uropathogenic Escherichia coli (UPEC) and diarrheagenic E.coli (DEC) strains to extend the knowledge of E.coli classification into different pathotypes. A total of 173 UPEC and DEC strains were examined for phylogenetic typing and urovirulence genes by PCR amplifications. In contrast to most reports, phylogenetic group A was the most prevalent in both UPEC and DEC strains, followed by B2 group. Amplification assays revealed that 89.32% and 94.29% of UPEC and DEC strains, respectively, carried at least one of the urovirulence genes, 49.5% and 31.4% of UPEC and DEC strains, respectively, carried ≥ 2 of the urovirulence genes, fim H gene was the most prevalent (66.9% and 91.4%) in UPEC and DEC strains respectively. Twenty different patterns of virulence genes were identified in UPEC while 5 different patterns in DEC strains. Strains with combined virulence patterns of four or five genes were belonged to phylogenetic group B2. Our finding showed a closer relationship between the DEC and UPEC, so raised the suggestion that some DEC strains might be potential uropathogens. These findings also provide different insights into the phylogenetic classification of E. coli as pathogenic or commensals where group A can be an important pathogenic type as well as into the classification as intestinal or extra- intestinal virulence factors.

Klíčová slova:

Biology and life sciences – Evolutionary biology – Evolutionary systematics – Phylogenetics – Animal phylogenetics – Taxonomy – Molecular biology – Molecular biology techniques – Artificial gene amplification and extension – Polymerase chain reaction – Microbiology – Medical microbiology – Microbial pathogens – Bacterial pathogens – Zoology – Anatomy – Digestive system – Gastrointestinal tract – Computer and information sciences – Data management – Medicine and health sciences – Pathology and laboratory medicine – Pathogens – Virulence factors – Diarrhea – Gastroenterology and hepatology – Diagnostic medicine – Signs and symptoms – Urology – Urinary tract infections – Research and analysis methods


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2019 Číslo 9

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