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Ontogenetic expression of thyroid hormone signaling genes: An in vitro and in vivo species comparison


Autoři: Kyla M. Walter aff001;  Katharina Dach aff002;  Keri Hayakawa aff001;  Susanne Giersiefer aff002;  Heike Heuer aff002;  Pamela J. Lein aff001;  Ellen Fritsche aff002
Působiště autorů: Department of Molecular Biosciences, University of California-Davis, School of Veterinary Medicine, Davis, CA, United States of America aff001;  IUF–Leibniz Research Institute for Environmental Medicine, Dusseldorf, Germany aff002;  Dept. Endocrinology, University Hospital Essen, Essen, Germany aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0221230

Souhrn

Thyroid hormone (TH) is essential for brain development. While disruption of TH signaling by environmental chemicals has been discussed as a mechanism of developmental neurotoxicity (DNT) for more than a decade, there remains a paucity of information linking specific TH disrupting chemicals to adverse neurodevelopmental outcomes. This data gap reflects, in part, the fact that the molecular machinery of TH signaling is complex and varies according to cell type and developmental time. Thus, establishing a baseline of the ontogenetic profile of expression of TH signaling molecules in relevant cell types is critical for developing in vitro and alternative systems-based models for screening TH disrupting chemicals for DNT. Here, we characterize the transcriptomic profile of molecules critical to TH signaling across three species–human, rat, and zebrafish–in vitro and in vivo across different stages of neurodevelopment. Our data indicate that while cultured human and rat neural progenitor cells, primary cultures of rat cortical cells, and larval zebrafish all express a fairly comprehensive transcriptome of TH signaling molecules, the spatiotemporal expression profiles as well as the responses to TH vary across species and developmental stages. The data presented here provides a roadmap for identifying appropriate in vitro and in simpler systems-based models for mechanistic studies and screening of chemicals that alter neurodevelopment via interference with TH action.

Klíčová slova:

Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Zebrafish – Animal models – Biological cultures – Cell cultures – Neurospheres – Extraction techniques – RNA extraction – Biology and life sciences – Organisms – Eukaryota – Animals – Vertebrates – Fish – Osteichthyes – Genetics – Gene expression – Developmental biology – Morphogenesis – Neurodevelopment – Embryology – Embryos – Life cycles – Larvae – Molecular biology – Molecular biology techniques – Artificial gene amplification and extension – Polymerase chain reaction


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