Microbiome profiling of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae)

Autoři: Suresh J. Gawande aff001;  Sivalingam Anandhan aff001;  Ashish Ingle aff001;  Praveen Roylawar aff001;  Kiran Khandagale aff001;  Tushar Gawai aff001;  Alana Jacobson aff002;  Ramasamy Asokan aff003;  Major Singh aff001
Působiště autorů: ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, India aff001;  Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama, United States of America aff002;  ICAR-Indian Institute of Horticultural Research, Hessarghatta Lake, Bengaluru, India aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223281


The gut microbial community structure of adult Thrips tabaci collected from 10 different agro-climatically diverse locations of India was characterized by using the Illumina MiSeq platform to amplify the V3 region of the 16S rRNA gene of bacteria present in the sampled insects. Analyses were performed to study the bacterial communities associated with Thrips tabaci in India. The complete bacterial metagenome of T. tabaci was comprised of 1662 OTUs of which 62.25% belong to known and 37.7% of unidentified/unknown bacteria. These OTUs constituted 21 bacterial phyla of 276 identified genera. Phylum Proteobacteria was predominant, followed by Actinobacteria, Firmicutes, Bacteroidetes and Cyanobacteria. Additionally, the occurrence of the reproductive endosymbiont, Wolbachia was detected at two locations (0.56%) of the total known OTUs. There is high variation in diversity and species richness among the different locations. Alpha-diversity metrics indicated the higher gut bacterial diversity at Bangalore and lowest at Rahuri whereas higher bacterial species richness at T. tabaci samples from Imphal and lowest at Jhalawar. Beta diversity analyses comparing bacterial communities between the samples showed distinct differences in bacterial community composition of T. tabaci samples from different locations. This paper also constitutes the first record of detailed bacterial communities associated with T. tabaci. The location-wise variation in microbial metagenome profile of T. tabaci suggests that bacterial diversity might be governed by its population genetic structure, environment and habitat.

Klíčová slova:

Actinobacteria – Bacteria – India – Microbiome – Ribosomal RNA – Sequence databases – Species diversity – Wolbachia


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