Malaria transmission through the mosquito requires the function of the OMD protein

Autoři: Chiara Currà aff001;  Jessica Kehrer aff002;  Leandro Lemgruber aff002;  Patricia A. G. C. Silva aff003;  Lucia Bertuccini aff004;  Fabiana Superti aff004;  Tomasino Pace aff006;  Marta Ponzi aff004;  Friedrich Frischknecht aff002;  Inga Siden-Kiamos aff001;  Gunnar R. Mair aff002
Působiště autorů: Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Greece aff001;  Integrative Parasitology, Center for Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany aff002;  Instituto Medicina Molecular, Lisbon, Portugal aff003;  Core Facilities, National Institute of Health, Rome, Italy aff004;  National Center for Innovative Technologies in Public Health, National Institute of Health, Rome, Italy aff005;  Department of Infectious Diseases, National Institute of Health, Rome, Italy aff006;  Iowa State University, Biomedical Sciences, Ames, Iowa, United States of America aff007
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222226


Ookinetes, one of the motile and invasive forms of the malaria parasite, rely on gliding motility in order to establish an infection in the mosquito host. Here we characterize the protein PBANKA_0407300 which is conserved in the Plasmodium genus but lacks significant similarity to proteins of other eukaryotes. It is expressed in gametocytes and throughout the invasive mosquito stages of P. berghei, but is absent from asexual blood stages. Mutants lacking the protein developed morphologically normal ookinetes that were devoid of productive motility although some stretching movement could be detected. We therefore named the protein Ookinete Motility Deficient (OMD). Several key factors known to be involved in motility however were normally expressed and localized in the mutant. Importantly, the mutant failed to establish an infection in the mosquito which resulted in a total malaria transmission blockade.

Klíčová slova:

Blood – Gametocytes – Malarial parasites – Parasitic diseases – Plasmid construction – Plasmodium – Polymerase chain reaction – Oocysts


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