Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet


Autoři: KaLynn Harlow aff001;  Christina R. Ferreira aff002;  Tiago J. P. Sobreira aff002;  Theresa Casey aff001;  Kara Stewart aff001
Působiště autorů: Department of Animal Sciences, Purdue University, West Lafayette, Indiana, United States of America aff001;  Metabolomics Core, Bindley Science Center, Purdue University, West Lafayette, Indiana, United States of America aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0215186

Souhrn

We hypothesized that postnatal development of the vagina is impacted by early nutritional environment. Our objective was to determine if lipid profiles of vaginal swabs were different between postnatal gilts suckled by sow or fed milk replacer the first 48 h after birth, with or without a lard-based fat supplement. Gilts (>1.3 kg) were selected at birth across 8 litters and assigned to one of four treatments: 1) suckled by sow (S, n = 8); 2) suckled by sow plus administration of a fat supplement (SF, n = 5); 3) bottle-fed solely milk replacer (B, n = 8); or 4) bottle-fed solely milk replacer plus administration of a fat supplement (BF, n = 7). At 48 h postnatal, vaginal swabs of gilts were taken with a cytology brush, and lipids were extracted for analysis using multiple reaction monitoring (MRM)-profiling. Lipids extracted from serum collected at 48 h from gilts, milk collected at 24 h from sows, and milk replacer were also analyzed with MRM-profiling. Receiver operating characteristic curve analysis found 18 lipids recovered from vaginal swabs that highly distinguished between S and B gilts [area-under-the-curve (AUC) > 0.9], including phosphatidylethanolamine with 34 carbons and four unsaturations in the fatty acyl residues [PE (34:4)]. Twelve lipids from vaginal swabs highly correlated (r > 0.6; p < 0.01) with nutrition source. Lipids with greater abundance in milk replacer drove association. For example, mean intensity of PE (34:4) was 149-fold higher in milk replacer than colostrum. Consequently, PE (34:4) was found to have 1.6- and 2.12-fold higher levels in serum and vaginal swab samples (p < 0.001), respectively, of B gilts as compared to S gilts. Findings support that vaginal swabs can be used to noninvasively study effects of perinatal nutrition on tissue composition.

Klíčová slova:

Breast milk – Fats – Lipids – Milk – Nutrition – Swine – Lipid analysis – Lipid profiles


Zdroje

1. Peaker M. The mammary gland in mammalian evolution: a brief commentary on some of the concepts. Journal of mammary gland biology and neoplasia 2002; 7:347–353. 12751896

2. Dividich JL, Rooke JA, Herpin P. Nutritional and immunological importance of colostrum for the new-born pig. J. Agric. Sci. 2005; 143:469–485.

3. Bartol FF, Wiley AA, Bagnell CA. Relaxin and Maternal Lactocrine Programming of Neonatal Uterine Development. Annals of the New York Academy of Sciences 2009; 1160:158–163. doi: 10.1111/j.1749-6632.2008.03820.x 19416179

4. Bagnell CA, Yan W, Wiley AA, Bartol FF. Effects of Relaxin on Neonatal Porcine Uterine Growth and Development. Annals of the New York Academy of Sciences 2005; 1041:248–255. doi: 10.1196/annals.1282.038 15956715

5. Gray CA, Bazer FW, Spencer TE. Effects of Neonatal Progestin Exposure on Female Reproductive Tract Structure and Function in the Adult Ewe1. Biology of Reproduction 2001; 64:797–804. doi: 10.1095/biolreprod64.3.797 11207194

6. Bartol FF, Wiley AA, Spencer TE, Vallet JL. Early uterine development in pigs. Journal of Reproduction and fertility supplement 1993; 48:99–116. 8145217

7. Yan W, Chen J, Wiley AA, Crean-Harris BD, Bartol FF, Bagnell CA. Relaxin (RLX) and estrogen affect estrogen receptor α, vascular endothelial growth factor, and RLX receptor expression in the neonatal porcine uterus and cervix. Reproduction 2008; 135:705–712. doi: 10.1530/REP-08-0014 18304981

8. Yan W, Wiley AA, Bathgate RA, Frankshun AL, Lasano S, Crean BD, et al. Expression of LGR7 and LGR8 by Neonatal Porcine Uterine Tissues and Transmission of Milk-Borne Relaxin into the Neonatal Circulation by Suckling. Endocrinology 2006; 147:4303–4310. doi: 10.1210/en.2006-0397 16740969

9. Camp ME, Wiley AA, Boulos MB, Rahman KM, Bartol FF, Bagnell CA. Effects of age, nursing, and oral IGF1 supplementation on neonatal porcine cervical development. Reproduction 2014; 148:441–451. doi: 10.1530/REP-14-0257 25074922

10. Miller DJ, Wiley AA, Chen JC, Bagnell CA, Bartol FF. Nursing for 48 Hours from Birth Supports Porcine Uterine Gland Development and Endometrial Cell Compartment-Specific Gene Expression. Biology of Reproduction 2013; 88.

11. Bartol FF, Wiley AA, Miller DJ, Silva AJ, Roberts KE, Davolt ML, et al. Lactation Biology Symposium: lactocrine signaling and developmental programming. Journal of animal science 2013; 91:696. doi: 10.2527/jas.2012-5764 23100582

12. Bartol FF, Wiley AA, Miller DJ, Silva AJ, Roberts KE, Davolt ML et al. LACTATION BIOLOGY SYMPOSIUM: Lactocrine signaling and developmental programming. Journal of Animal Science 2013; 91:696–705. doi: 10.2527/jas.2012-5764 23100582

13. Chen JC, Frankshun AL, Wiley AA, Miller DJ, Welch KA, Ho TY, et al. Milk-borne lactocrine-acting factors affect gene expression patterns in the developing neonatal porcine uterus. Reproduction 2011; 141:675–683. doi: 10.1530/REP-10-0320 21317299

14. Cai Y. Revisiting old vaginal topics: conversion of the Müllerian vagina and origin of the" sinus" vagina. International Journal of Developmental Biology 2009; 53:925–934. doi: 10.1387/ijdb.082846yc 19598112

15. Casey T, Harlow K, Ferreira CR, Sobreira TJP, Schinckel A, Stewart K. The potential of identifying replacement gilts by screening for lipid biomarkers in reproductive tract swabs taken at weaning. Journal of Applied Animal Research 2018; 46:667–676.

16. Casey T, Harlow K, Ferreira CR, Sobreira TJ, Schinckel A, Stewart K. The potential of identifying replacement gilts by screening for lipid biomarkers in reproductive tract swabs taken at weaning. Journal of Applied Animal Research 2018; 46:667–676.

17. Benevenga N, Steinman-Goldsworthy J, Crenshaw T, Odle J. Utilization of Medium-Chain Triglycerides by Neonatal Piglets: I. Effects on Milk Consumption and Body Fuel Utilization1. Journal of animal science 1989; 67:3331–3339. doi: 10.2527/jas1989.67123331x 2613579

18. Odle J, Benevenga N, Crenshaw T. Utilization of medium-chain triglycerides by neonatal piglets: II. Effects of even-and odd-chain triglyceride consumption over the first 2 days of life on blood metabolites and urinary nitrogen excretion. Journal of Animal Science 1989; 67:3340–3351. doi: 10.2527/jas1989.67123340x 2613580

19. Le Dividich J. Effect of fat content of colostrum on voluntary colostrum intake and fat utilization in newborn pigs. Journal Of Animal Science 1997; 75:707. doi: 10.2527/1997.753707x 9078487

20. Su G, Zhou X, Wang Y, Chen D, Chen G, Li Y, He J. Effects of plant essential oil supplementation on growth performance, immune function and antioxidant activities in weaned pigs. Lipids Health Dis 2018; 17:139. doi: 10.1186/s12944-018-0788-3 29903022

21. Wieland TM, Lin X, Odle J. Utilization of medium-chain triglycerides by neonatal pigs: effects of emulsification and dose delivered. Journal of animal science 1993; 71:1863–1868. doi: 10.2527/1993.7171863x 8349513

22. Bligh E, Dyer W. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917. doi: 10.1139/o59-099 13671378

23. Chong J, Soufan O, Li C, Caraus I, Li S, Bourque G, Wishart DS, Xia J. MetaboAnalyst 4.0: towards more transparent and integrative metabolomics analysis. Nucleic acids research 2018.

24. Faraggi D, Reiser B, Schisterman EF. ROC curve analysis for biomarkers based on pooled assessments. Statistics in Medicine 2003; 22:2515–2527. doi: 10.1002/sim.1418 12872306

25. Xia J, Broadhurst D, Wilson M, Wishart D. Translational biomarker discovery in clinical metabolomics: an introductory tutorial. Metabolomics 2013; 9:280–299. doi: 10.1007/s11306-012-0482-9 23543913

26. Forman BM, Tontonoz P, Chen J, Brun RP, Spiegelman BM, Evans RM. 15-Deoxy-Δ12,14-Prostaglandin J2 is a ligand for the adipocyte determination factor PPARγ. Cell 1995; 83:803–812. doi: 10.1016/0092-8674(95)90193-0 8521497

27. Kliewer SA, Sundseth SS, Jones SA, Brown PJ, Wisely GB, Koble CS, et al. Fatty acids and eicosanoids regulate gene expression through direct interactions with peroxisome proliferator-activated receptors α and γ. Proceedings of the National Academy of Sciences 1997; 94:4318–4323.

28. Delplanque B, Gibson R, Koletzko B, Lapillonne A, Strandvik B. Lipid Quality in Infant Nutrition: Current Knowledge and Future Opportunities. Journal of pediatric gastroenterology and nutrition 2015; 61:8–17. doi: 10.1097/MPG.0000000000000818 25883056

29. Lanza I, Shoup DI, Saif LJ. Lactogenic immunity and milk antibody isotypes to transmissible gastroenteritis virus in sows exposed to porcine respiratory coronavirus during pregnancy. Am J Vet Res 1995; 56:739–748. 7653882

30. Drew MD, Bevandick I, Owen BD. Artificial rearing of colostrum-deprived piglets using iron chelators: The effects of oral administration of EDDHA with and without bovine or porcine immunoglobulins on piglet performance and iron-metabolism. Can. J. Anim. Sci. 1990; 70:655–666.

31. Bourne FJ, Newby TJ, Evans P, Morgan K. The immune requirements of the newborn pig and calf. Ann Rech Vet 1978; 9:239–244. 371505

32. Davidson MH, Johnson J, Rooney MW, Kyle ML, Kling DF. A novel omega-3 free fatty acid formulation has dramatically improved bioavailability during a low-fat diet compared with omega-3-acid ethyl esters: the ECLIPSE (Epanova® compared to Lovaza® in a pharmacokinetic single-dose evaluation) study. Journal of clinical lipidology 2012; 6:573–584. doi: 10.1016/j.jacl.2012.01.002 23312053

33. Hamosh M, Bitman J, Wood DL, Hamosh P, Mehta N. Lipids in milk and the first steps in their digestion. Pediatrics 1985; 75:146–150. 3880885


Článek vyšel v časopise

PLOS One


2019 Číslo 9

Nejčtenější v tomto čísle

Tomuto tématu se dále věnují…


Kurzy

Zvyšte si kvalifikaci online z pohodlí domova

Krvácení v důsledku portální hypertenze při jaterní cirhóze – od pohledu záchranné služby až po závěrečný hepato-gastroenterologický pohled
nový kurz
Autoři: PhDr. Petr Jaššo, MBA, MUDr. Hynek Fiala, Ph.D., prof. MUDr. Radan Brůha, CSc., MUDr. Tomáš Fejfar, Ph.D., MUDr. David Astapenko, Ph.D., prof. MUDr. Vladimír Černý, Ph.D.

Rozšíření možností lokální terapie atopické dermatitidy v ordinaci praktického lékaře či alergologa
Autoři: MUDr. Nina Benáková, Ph.D.

Léčba bolesti v ordinaci praktického lékaře
Autoři: MUDr. PhDr. Zdeňka Nováková, Ph.D.

Revmatoidní artritida: včas a k cíli
Autoři: MUDr. Heřman Mann

Jistoty a nástrahy antikoagulační léčby aneb kardiolog - neurolog - farmakolog - nefrolog - právník diskutují
Autoři: doc. MUDr. Štěpán Havránek, Ph.D., prof. MUDr. Roman Herzig, Ph.D., doc. MUDr. Karel Urbánek, Ph.D., prim. MUDr. Jan Vachek, MUDr. et Mgr. Jolana Těšínová, Ph.D.

Všechny kurzy
Kurzy Doporučená témata Časopisy
Přihlášení
Zapomenuté heslo

Nemáte účet?  Registrujte se

Zapomenuté heslo

Zadejte e-mailovou adresu se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se