In vivo clearance of nanoparticles by transcytosis across alveolar epithelial cells

Autoři: Pascal Detampel aff001;  Anutosh Ganguly aff002;  Sara Tehranian aff004;  Francis Green aff005;  Santiswarup Singha aff002;  Pere Santamaria aff002;  Ayodeji A. Jeje aff004;  Clifford S. Cho aff003;  Björn Petri aff002;  Matthias W. Amrein aff001
Působiště autorů: Department of Cell Biology and Anatomy, University of Calgary, Calgary, Canada aff001;  Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Canada aff002;  Department of Surgery, University of Michigan at Ann Arbor, Ann Arbor, Michigan, United States of America aff003;  Department of Chemical and Petroleum Engineering, University of Calgary, Calgary, Canada aff004;  Department Pathology & Laboratory Medicine, University of Calgary, Calgary, Canada aff005;  The Snyder Institute for Chronic Diseases, University of Calgary, Calgary, Canada aff006
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223339


Nanoparticles in polluted air or aerosolized drug nanoparticles predominantly settle in the alveolar lung. Here, we describe a novel, highly effective pathway for the particles to cross the alveolar epithelium and reach the lymph and bloodstream. Amorphous silica nanoparticles, suspended in perfluorocarbon, were instilled into the lungs of mice for intravital microscopy. Particles formed agglomerates that settled on the alveolar wall, half of which were removed from the lung within 30 minutes. TEM histology showed agglomerates in stages of crossing the alveolar epithelium, in large compartments inside the epithelial cells and crossing the basal membrane into the interstitium. This pathway is consistent with published kinetic studies in rats and mice, using a host of (negatively) charged and polar nanoparticles.

Klíčová slova:

Body weight – Epithelial cells – Epithelium – Fluorescence microscopy – Nanoparticles – Pulmonary imaging – Transmission electron microscopy – Alveolar macrophages


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