Identification of QTLs for powdery mildew (Podosphaera aphanis; syn. Sphaerotheca macularis f. sp. fragariae) susceptibility in cultivated strawberry (Fragaria ×ananassa)


Autoři: Daniel J. Sargent aff001;  Matteo Buti aff003;  Nada Šurbanovski aff001;  May Bente Brurberg aff004;  Muath Alsheikh aff005;  Matthew P. Kent aff007;  Jahn Davik aff004
Působiště autorů: PlantSci Consulting Ltd. Kent, United Kingdom aff001;  Fondazione Edmund Mach, San Michele all’Adige, Trentino, Italy aff002;  Department of Agriculture, Food, Environment and Forestry, University of Florence, Florence, Italy aff003;  Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, Norway aff004;  Department of Plant Sciences, Norwegian University of Life Sciences, Ås, Norway aff005;  Graminor Breeding Ltd., Ridabu, Norway aff006;  Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Ås, Norway aff007
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222829

Souhrn

Strawberry powdery mildew (Podosphaera aphanis Wallr.) is a pathogen which infects the leaves, fruit, stolon and flowers of the cultivated strawberry (Fragaria ×ananassa), causing major yield losses, primarily through unmarketable fruit. The primary commercial control of the disease is the application of fungicidal sprays. However, as the use of key active ingredients of commercial fungicides is becoming increasingly restricted, interest in developing novel strawberry cultivars exhibiting resistance to the pathogen is growing rapidly. In this study, a mapping population derived from a cross between two commercial strawberry cultivars (‘Sonata’ and ‘Babette’) was genotyped with single nucleotide polymorphism (SNP) markers from the Axiom iStraw90k genotyping array and phenotyped for powdery mildew susceptibility in both glasshouse and field environments. Three distinct, significant QTLs for powdery mildew resistance were identified across the two experiments. Through comparison with previous studies and scrutiny of the F. vesca genome sequence, candidate genes underlying the genetic control of this trait were identified.

Klíčová slova:

Biology and life sciences – Genetics – Genetic loci – Quantitative trait loci – Genomics – Heredity – Genetic linkage – Plant science – Plant pathology – Plant pathogens – Plant fungal pathogens – Powdery mildew – Plant anatomy – Leaves – Molecular biology – Molecular biology techniques – Gene mapping – Linkage mapping – Research and analysis methods – Database and informatics methods – Bioinformatics – Sequence analysis


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PLOS One


2019 Číslo 9

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