Identification and evolutionary characterization of salt-responsive transcription factors in the succulent halophyte Suaeda fruticosa


Autoři: Joann Diray-Arce aff001;  Alisa Knowles aff001;  Anton Suvorov aff002;  Jacob O’Brien aff001;  Collin Hansen aff001;  Seth M. Bybee aff002;  Bilquees Gul aff003;  M. Ajmal Khan aff003;  Brent L. Nielsen aff001
Působiště autorů: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America aff001;  Department of Biology, Brigham Young University, Provo, Utah, United States of America aff002;  Institute of Sustainable Halophyte Utilization, University of Karachi, Karachi, Pakistan aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222940

Souhrn

Transcription factors are key regulatory elements that affect gene expression in response to specific signals, including environmental stresses such as salinity. Halophytes are specialized plants that have the ability to complete their life cycle in saline environments. In this study we have identified and characterized the evolutionary relationships of putative transcription factors (TF) in an obligate succulent halophyte, Suaeda fruticosa, that are involved in conferring salt tolerance. Using RNA-seq data we have analyzed the expression patterns of certain TF families, predicted protein-protein interactions, and analyzed evolutionary trajectories to elucidate their possible roles in salt tolerance. We have detected the top differentially expressed (DE) transcription factor families (MYB, CAMTA, MADS-box and bZIP) that show the most pronounced response to salinity. The majority of DE genes in the four aforementioned TF families cluster together on TF phylogenetic trees, which suggests common evolutionary origins and trajectories. This research represents the first comprehensive TF study of a leaf succulent halophyte including their evolutionary relationships with TFs in other halophyte and salt-senstive plants. These findings provide a foundation for understanding the function of salt-responsive transcription factors in salt tolerance and associated gene regulation in plants.

Klíčová slova:

DNA transcription – Gene expression – Multiple alignment calculation – Phylogenetic analysis – Plant resistance to abiotic stress – Sequence alignment – Transcription factors – Quinoa


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