Genetic relatedness in carbapenem-resistant isolates from clinical specimens in Ghana using ERIC-PCR technique
Autoři:
Francis S. Codjoe aff001; Charles A. Brown aff001; Thomas J. Smith aff002; Keith Miller aff002; Eric S. Donkor aff003
Působiště autorů:
Department of Medical Laboratory Sciences, School of Biomedical & Allied Health Sciences, College of Health Sciences, University of Ghana, Ghana
aff001; Biomolecular Science Research Centre, Sheffield Hallam University, Sheffield, England, United Kingdom
aff002; Department of Medical Microbiology, School of Biomedical & Allied Health Sciences, College of Health Sciences, University of Ghana, Ghana
aff003
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0222168
Souhrn
Aim
Enterobacterial repetitive intergenic consensus (ERIC) sequence analysis is a powerful tool for epidemiological analysis of bacterial species. This study aimed to determine the genetic relatedness or variability in carbapenem-resistant isolates by species using this technique.
Methods
A total of 111 non-duplicated carbapenem-resistant (CR) Gram-negative bacilli isolates from a three-year collection period (2012–2014) were investigated by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC–PCR) in four selected hospital laboratories in Ghana. The isolates were also screened for carbapenemase and extended spectrum β-lactamase genes by PCR.
Results
A proportion of 23.4% (26/111) of the genomic DNA extracts were carriers of PCR-positive carbapenemase genes, including 14.4% blaNDM-1, 7.2% blaVIM-1 and 1.8% blaOXA-48. The highest prevalence of carbapenemase genes was from non-fermenters, Acinetobacter baumannii and Pseudomonas aeruginosa. For the ESBL genes tested, 96.4% (107/111) of the CR isolates co-harboured both TEM-1 and SHV-1 genes. The ERIC-PCR gel analysis exhibited 1 to 8 bands ranging from 50 to 800 bp. Band patterns of 93 complex dissimilarities were visually distinguished from the 111 CR isolates studied, while the remaining 18 showed band similarities in pairs.
Conclusion
Overall, ERIC-PCR fingerprints have shown a high level of diversity among the species of Gram-negative bacterial pathogens and specimen collection sites in this study. ERIC-PCR optimisation assays may serve as a suitable genotyping tool for the assessment of genetic diversity or close relatedness of isolates that are found in clinical settings.
Klíčová slova:
Biology and life sciences – Microbiology – Medical microbiology – Microbial pathogens – Bacterial pathogens – Pseudomonas aeruginosa – Acinetobacter – Acinetobacter baumannii – Microbial control – Organisms – Bacteria – Pseudomonas – Molecular biology – Molecular biology techniques – Genetic fingerprinting and footprinting – Genetic fingerprinting – Artificial gene amplification and extension – Polymerase chain reaction – Medicine and health sciences – Pathology and laboratory medicine – Pathogens – Pharmacology – Antimicrobial resistance – Infectious diseases – Bacterial diseases – Acinetobacter infections – Pseudomonas infections – Research and analysis methods
Zdroje
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Článek vyšel v časopise
PLOS One
2019 Číslo 9
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