Exploring the microbiota of upper respiratory tract during the development of pneumonia in a mouse model


Autoři: Yoshitomo Morinaga aff001;  Yuki Take aff001;  Daisuke Sasaki aff001;  Kenji Ota aff001;  Norihito Kaku aff001;  Naoki Uno aff001;  Kei Sakamoto aff001;  Kosuke Kosai aff001;  Taiga Miyazaki aff002;  Hiroo Hasegawa aff001;  Koichi Izumikawa aff002;  Hiroshi Mukae aff002;  Katsunori Yanagihara aff001
Působiště autorů: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Nagasaki, Japan aff001;  Department of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Nagasaki, Japan aff002;  Department of Infectious Diseases, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Nagasaki, Japan aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222589

Souhrn

The alteration of the microbial community in the upper respiratory tract (URT) can contribute to the colonization and invasion of respiratory pathogens. However, there are no studies regarding whether the characteristics of the URT microbiota can be affected by infections in lower respiratory tract (LRT). To elucidate the microbial profiles of the URT during pneumonia, the oral, nasal, and lung microbiota was evaluated at the early phase in a murine pneumonia model by direct intratracheal inoculation of Klebsiella pneumoniae. The meta 16S rRNA sequencing of bronchoalveolar lavage fluid after K. pneumoniae inoculation presented alterations in the beta diversity of the microbes, but not in the alpha diversity. At this point, a significant increase in microbial alpha diversity was observed in the oral cavity, but not in the nasal cavity. The significant increase was observed in the family Carnobacteriaceae and family Enterococcaceae. These results suggest that characterizing the microbial community of the respiratory tract may not just involve a simple downstream relationship from the URT to the LRT. The health status of the LRT may influence the oral microbiota. Thus, evaluation of the oral microbiota may contribute towards monitoring lung health; the oral microbiota may act as a diagnostic marker of pneumonia.

Klíčová slova:

Bacterial pathogens – Klebsiella pneumoniae – Microbiome – Mouse models – Pneumonia – Respiratory infections – Respiratory physiology – Streptococcal infections


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