Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections

English version

Autoři: Theresa Schwaiger ^aff001; Julia Sehl ^aff001; Claudia Karte ^aff003; Alexander Schäfer ^aff004; Jane Hühr ^aff004; Thomas C. Mettenleiter ^aff002; Charlotte Schröder ^aff001; Bernd Köllner ^aff004; Reiner Ulrich ^aff001; Ulrike Blohm ^aff004
Působiště autorů: Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany ^aff001; Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany ^aff002; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany ^aff003; Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany ^aff004; Institute of Veterinary Pathology, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany ^aff005
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0222943

Souhrn

Pigs are anatomically, genetically and physiologically comparable to humans and represent a natural host for influenza A virus (IAV) infections. Thus, pigs may represent a relevant biomedical model for human IAV infections. We set out to investigate the systemic as well as the local immune response in pigs upon two subsequent intranasal infections with IAV H1N1pdm09. We detected decreasing numbers of peripheral blood lymphocytes after the first infection. The simultaneous increase in the frequencies of proliferating cells correlated with an increase in infiltrating leukocytes in the lung. Enhanced perforin expression in αβ and γδ T cells in the respiratory tract indicated a cytotoxic T cell response restricted to the route of virus entry such as the nose, the lung and the bronchoalveolar lavage. Simultaneously, increasing frequencies of CD8αα expressing αβ T cells were observed rapidly after the first infection, which may have inhibited uncontrolled inflammation in the respiratory tract. Taking together, the results of this study demonstrate that experimental IAV infection in pigs mimics major characteristics of human seasonal IAV infections.

Klíčová slova:

Biology and life sciences – Cell biology – Cellular types – Animal cells – Blood cells – White blood cells – T cells – Cytotoxic T cells – Immune cells – Organisms – Eukaryota – Animals – Vertebrates – Amniotes – Mammals – Swine – Viruses – RNA viruses – Orthomyxoviruses – Influenza viruses – Influenza A virus – Microbiology – Medical microbiology – Microbial pathogens – Viral pathogens – Anatomy – Head – Face – Nose – Lymphatic system – Lymph nodes – Medicine and health sciences – Immunology – Immune response – Pathology and laboratory medicine – Pathogens – Pulmonology – Respiratory infections

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Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections

Souhrn

Klíčová slova:

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