Effects of salbutamol and phlorizin on acute pulmonary inflammation and disease severity in experimental sepsis
Autoři:
Léia Cardoso-Sousa aff001; Emilia Maria Gomes Aguiar aff001; Douglas Carvalho Caixeta aff002; Danielle Diniz Vilela aff002; Danilo Pereira da Costa aff001; Tamires Lopes Silva aff003; Thúlio Marquez Cunha aff004; Paulo Rogério Faria aff005; Foued Salmen Espindola aff002; Ana Carolina Jardim aff006; Alexandre Antônio Vieira aff001; Tales Lyra Oliveira aff007; Luiz Ricardo Goulart aff002; Robinson Sabino-Silva aff001
Působiště autorů:
Department of Physiology, Institute of Biomedical Sciences, Federal University of Uberlandia, Minas Gerais, Brazil
aff001; Institute of Biotechnology, Federal University of Uberlandia, Minas Gerais, Brazil
aff002; Institute of Biomedical Sciences, Laboratory of Immunoparasitology "Dr. Mario Endsfeldz Camargo", Federal University of Uberlandia, Minas Gerais, Brazil
aff003; Department of Pulmonology, School of Medicine, Federal University of Uberlandia, Minas Gerais, Brazil
aff004; Department of Morphology, Institute of Biomedical Sciences, Federal University of Uberlandia, Minas Gerais, Brazil
aff005; Laboratory of Virology, Institute of Biomedical Sciences, Federal University of Uberlandia, Minas Gerais, Brazil
aff006; Faculty of Medicine, Municipal University of Sao Caetano do Sul, Sao Paulo, Brazil
aff007; Department of Medical Microbiology and Immunology, University of California Davis, California, United States of America
aff008
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0222575
Souhrn
Respiratory infection can be exacerbated by the high glucose concentration in the airway surface liquid (ASL). We investigated the effects of salbutamol and phlorizin on the pulmonary function, oxidative stress levels and SGLT1 activity in lung, pulmonary histopathological damages and survival rates of rats with sepsis. Sepsis was induced by cecal ligation and puncture surgery (CLP). Twenty-four hours after surgery, CLP rats were intranasally treated with saline, salbutamol or phlorizin. After 2 hours, animals were anesthetized and sacrificed. Sepsis promoted atelectasis and bronchial inflammation, and led to increased expression of SGLT1 on cytoplasm of pneumocytes. Salbutamol treatment reduced bronchial inflammation and promoted hyperinsuflation in CLP rats. The interferon-ɤ and Interleucin-1β concentrations in bronchoalveolar lavage (BAL) were closely related to the bronchial inflammation regulation. Salbutamol stimulated SGLT1 in plasma membrane; whereas, phlorizin promoted the increase of SGLT1 in cytoplasm. Phlorizin reduced catalase activity and induced a significant decrease in the survival rate of CLP rats. Taken together, sepsis promoted atelectasis and lung inflammation, which can be associated with SGLT1 inhibition. The loss of function of SGLT1 by phlorizin are related to the augmented disease severity, increased atelectasis, bronchial inflammation and a significant reduction of survival rate of CLP rats. Alternatively salbutamol reduced BAL inflammatory cytokines, bronchial inflammation, atelectasis, and airway damage in sepsis. These data suggest that this selective β2-adrenergic agonist may protect lung of septic acute effects.
Klíčová slova:
Medicine and health sciences – Diagnostic medicine – Signs and symptoms – Sepsis – Pathology and laboratory medicine – Immunology – Immune response – Inflammation – Immune system – Innate immune system – Cytokines – Immune physiology – Biology and life sciences – Physiology – Developmental biology – Molecular development – Cell biology – Cellular structures and organelles – Cell membranes – Oxidative stress – Biochemistry – Enzymology – Enzymes – Catalases – Dismutases – Superoxide dismutase – Proteins – Physical sciences – Chemistry – Chemical compounds – Organic compounds – Carbohydrates – Monosaccharides – Glucose – Organic chemistry
Zdroje
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Článek vyšel v časopise
PLOS One
2019 Číslo 9
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