Downregulation of hippocampal NR2A/2B subunits related to cognitive impairment in a pristane-induced lupus BALB/c mice


Autoři: Jonatan Luciano-Jaramillo aff001;  Flavio Sandoval-García aff001;  Mónica Vázquez-Del Mercado aff001;  Yanet Karina Gutiérrez-Mercado aff006;  Rosa Elena Navarro-Hernández aff001;  Erika Aurora Martínez-García aff001;  Oscar Pizano-Martínez aff001;  Fernanda Isadora Corona-Meraz aff001;  Jacinto Bañuelos-Pineda aff008;  Jorge Fernando Floresvillar-Mosqueda aff009;  Beatriz Teresita Martín-Márquez aff001
Působiště autorů: Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Departamento de Biología Molecular y Genómica, Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético (IIRSME), Guadalajara, Jalisco, CP, México aff001;  Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Departamento de Clínicas Médicas, Guadalajara, Jalisco, CP, México aff002;  Universidad de Guadalajara, Envejecimiento, inmuno-metabolismo y estrés oxidativo, Guadalajara, Jalisco, CP, México aff003;  Hospital Civil de Guadalajara, Dr. Juan I. Menchaca, División de Medicina Interna, Servicio de Reumatología, Guadalajara, Jalisco, CP, México aff004;  Universidad de Guadalajara, Inmunología y Reumatología, Guadalajara, Jalisco, CP, México aff005;  Unidad de Evaluación Preclínica, Biotecnología Médica y Farmacéutica, CONACYT Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara, CP, México aff006;  Universidad de Guadalajara, Centro Universitario de Tonalá, Departamento de Ciencias Biomédicas, División de Ciencias de la Salud, Tonalá, Jalisco, CP, México aff007;  Universidad de Guadalajara, Centro Universitario de Ciencias Biológicas y Agropecuarias, Departamento de Medicina Veterinaria, Zapopan, Jalisco, CP, México aff008;  Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Departamento de Microbiología, Guadalajara, Jalisco, CP, México aff009
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0217190

Souhrn

Neuropsychiatric systemic lupus erythematosus (NPSLE) is associated with learning and memory deficit. Murine model of lupus induced by pristane in BALB/c mice is an experimental model that resembles some clinical and immunological SLE pathogenesis. Nevertheless, there is no experimental evidence that relates this model to cognitive dysfunction associated with NR2A/2B relative expression. To evaluate cognitive impairment related to memory deficits in a murine model of lupus induced by pristane in BALB/c mice related to mRNA relative expression levels of NR2A/2B hippocampal subunits in short and long-term memory task at 7 and 12 weeks after LPS exposition in a behavioral test with the use of Barnes maze. A total of 54 female BALB/c mice 8–12 weeks old were included into 3 groups: 7 and 12 weeks using pristane alone (0.5 mL of pristane) by a single intraperitoneal (i.p.) injection. A control group (single i.p. injection of 0.5 mL NaCl 0.9%) and pristane plus LPS exposure using single i.p. pristane injection and LPS of E. coli O55:B5, in a dose of 3mg/kg diluted in NaCl 0.9% 16 weeks post-pristane administration. To determine cognitive dysfunction, mice were tested in a Barnes maze. Serum anti-Sm antibodies and relative expression of hippocampal NR2A/2B subunits (GAPDH as housekeeping gene) with SYBR green quantitative reverse transcription polymerase chain reaction and 2-ΔΔCT method were determined in the groups. Downregulation of hippocampal NR2A subunit was more evident than NR2B in pristane and pristane+LPS at 7 and 12 weeks of treatment and it is related to learning and memory disturbance assayed by Barnes maze. This is the first report using the murine model of lupus induced by pristane that analyzes the NMDA subunit receptors, finding a downregulation of NR2A subunit related to learning and memory disturbance being more evident when they were exposed to LPS.

Klíčová slova:

Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Mouse models – Animal models – Immunologic techniques – Immunoassays – Enzyme-linked immunoassays – Biology and life sciences – Organisms – Eukaryota – Animals – Vertebrates – Amniotes – Mammals – Rodents – Mice – Neuroscience – Cognitive science – Cognitive neuroscience – Cognitive psychology – Learning – Learning and memory – Antibodies – Physiology – Biochemistry – Proteins – Immune system proteins – Autoantibodies – Psychology – Behavior – Animal behavior – Zoology – Medicine and health sciences – Neurology – Cognitive neurology – Cognitive impairment – Clinical medicine – Clinical immunology – Autoimmune diseases – Lupus erythematosus – Systemic lupus erythematosus – Immunology – Rheumatology – Immune physiology – Social sciences


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PLOS One


2019 Číslo 9