DNA analysis of elasmobranch products originating from Bangladesh reveals unregulated elasmobranch fishery and trade on species of global conservation concern

Autoři: Alifa Bintha Haque aff001;  Sudipta Arka Das aff003;  Aparna Riti Biswas aff001
Působiště autorů: Department of Zoology, University of Dhaka, Dhaka, Bangladesh aff001;  Department of Zoology, University of Oxford, Oxford, United Kingdom aff002;  Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222273


Trade involving elasmobranch products in Bangladesh is a four-decade-long practice in large scale and there is little understanding of its impact on species composition, population, and subsequent conservation. Capacity for monitoring and identification is lacking in landing and shark processing centres. A rapid survey and collection of tissue samples were performed in three landings and nine shark processing centres between 2016 and 2017 in the south-eastern coastal region of Bangladesh. Sequencing for a 707-bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was used to assess the taxonomic status and species composition from 71 elasmobranch tissue samples collected from the shark processing centre only. Good quality COI sequences were obtained for 34 specimens representing 21 species—the majority of which are threatened with extinction. A total of ten species of sharks (Carcharhinus brevipinna, C. amboinensis, C. leucas, C. sorrah, C. amblyrhynchoides, Chiloscyllium burmensis, Galeocerdo cuvier, Rhincodon typus, Scoliodon laticaudus, and Sphyrna lewini), eleven species of rays (Aetomylaeus maculatus, Gymnura poecilura, Mobula mobular, M. kuhlii, Neotrygon indica, Pateobatis uarnacoides, Rhinoptera javanica, and R. jayakari), including three species of guitarfish (Glaucostegus granulatus, G. obtusus, and G. typus), were identified. Four species (14.7% of samples) were found to be listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) in Appendix II. Sixteen species (59% of the specimens) were threatened with extinction according to IUCN Red List, whereas 41% were data deficient or not assessed. The results have important implications for the management of regional fisheries and the conservation of elasmobranchs as they 1) represent a preliminary understanding of elasmobranch diversity in trade; 2) depict a lack of awareness and monitoring; and 3) demonstrate a need for urgent monitoring and regulation of elasmobranch trade in Bangladesh.

Klíčová slova:

Bangladesh – BLAST algorithm – Elasmobranchii – Sequence alignment – Sequence databases – Sharks – Wildlife – Law enforcement


1. Schindler DE, Essington TE, Kitchell JF, Boggs C, Hilborn R. Sharks and tunas: fisheries impacts on predators with contrasting life histories. Ecol Appl. 2002 Jun;12(3):735–48.

2. Clarke SC, McAllister MK, Milner‐Gulland EJ, Kirkwood GP, Michielsens CG, Agnew DJ, et al. Global estimates of shark catches using trade records from commercial markets. Ecol Lett. 2006a Oct;9(10):1115–26

3. Clarke SC, Magnussen JE, Abercrombie DL, McAllister MK, Shivji MS. Identification of shark species composition and proportion in the Hong Kong shark fin market based on molecular genetics and trade records. Conserv Biol. 2006b Feb;20(1):201–11.

4. Dulvy NK, Baum JK, Clarke S, Compagno LJ, Cortés E, Domingo A, et al. You can swim but you can't hide: the global status and conservation of oceanic pelagic sharks and rays. Aquat Conserv. 2008 Jul 1;18(5):459–82.

5. Graham NA, Spalding MD, Sheppard CR. Reef shark declines in remote atolls highlight the need for multi‐faceted conservation action. Aquat Conserv. 2010 Jul;20(5):543–8.

6. Morgan A, Carlson JK. Capture time, size and hooking mortality of bottom longline-caught sharks. Fish Res. 2010 Jan 5;101(1–2):32–7.

7. Camhi MD, Valenti SV, Fordham SV, Fowler SL, Gibson C. The conservation status of pelagic sharks and rays: report of the IUCN shark specialist group pelagic shark red list workshop. IUCN Species Survival Commission Shark Specialist Group. Newbury, UK. x+ 78p. 2009.

8. Bräutigam A, Callow M, Campbell IR, Camhi MD, Cornish AS, Dulvy NK, et al. Global Priorities for Conserving Sharks and Rays. 2015.

9. Dulvy NK, Fowler SL, Musick JA, Cavanagh RD, Kyne PM, Harrison LR, et al. Extinction risk and conservation of the world’s sharks and rays. eLife. 2014 Jan 21;3:e00590. doi: 10.7554/eLife.00590 24448405

10. Jabado RW, Kyne PM, Pollom RA, Ebert DA, Simpfendorfer CA, Ralph GM, et al. Troubled waters: Threats and extinction risk of the sharks, rays and chimaeras of the Arabian Sea and adjacent waters. Fish Fish. 2018b.

11. Henderson AC, McIlwain JL, Al-Oufi HS, Al-Sheili S. The Sultanate of Oman shark fishery: Species composition, seasonality and diversity. Fish Res. 2007 Sep 1;86(2–3):159–68.

12. Moore AB. Elasmobranchs of the Persian (Arabian) Gulf: ecology, human aspects and research priorities for their improved management. Rev Fish Biol Fish. 2012 Mar 1;22(1):35–61.

13. Jabado RW, Al Ghais SM, Hamza W, Henderson AC. The shark fishery in the United Arab Emirates: an interview based approach to assess the status of sharks. Aquat Conserv. 2015 Dec;25(6):800–16.

14. Dent F, Clarke S. State of the global market for shark products. FAO Fisheries and Aquaculture technical paper. 2015(590):I.

15. Steinke D, Bernard AM, Horn RL, Hilton P, Hanner R, Shivji MS. DNA analysis of traded shark fins and mobulid gill plates reveals a high proportion of species of conservation concern. Sci Rep. 2017 Aug 25;7(1):9505. doi: 10.1038/s41598-017-10123-5 28842669

16. Spaet JL, Berumen ML. Fish market surveys indicate unsustainable elasmobranch fisheries in the Saudi Arabian Red Sea. Fish Res. 2015 Jan 1; 161:356–64.

17. Cardeñosa D, Fields A, Abercrombie D, Feldheim K, Shea SK, Chapman DD. A multiplex PCR mini-barcode assay to identify processed shark products in the global trade. PloS one. 2017 Oct 11;12(10): e0185368. doi: 10.1371/journal.pone.0185368 29020095

18. Haque AB, Biswas A, Latifa GA. Observations of shark and ray products in the processing centres of Bangladesh, trade in CITES species and conservation need. TRAFFIC Bulletin. 2018; 30(1):6–14.

19. Fischer J, Erikstein K, D'Offay B, Guggisberg S, Barone M. Review of the Implementation of the International Plan of Action for the Conservation and Management of Sharks. FAO Fisheries and Aquaculture Circular. 2012(C1076): I.

20. Henderson AC, Reeve AJ, Jabado RW, Naylor GJ. Taxonomic assessment of sharks, rays and guitarfishes (Chondrichthyes: Elasmobranchii) from south-eastern Arabia, using the NADH dehydrogenase subunit 2 (NADH2) gene. Zool J Linn Soc. 2016 Jan 25;176(2):399–442.

21. Hoq ME, Haroon AY, Hussain MG (Eds). Shark fisheries in the Bay of Bengal, Bangladesh: Status and potentialities. Support to Sustainable Management of the BOBLME Project, Bangladesh Fisheries Research Institute; 2011 Jan.

22. Akhilesh KV, Bineesh KK, Gopalakrishnan A, Jena JK, Basheer VS, Pillai NGK. Checklist of Chondrichthyans in Indian waters. 2014.

23. FRSS. Yearbook of Fisheries Statistics of Bangladesh. Fisheries Resources Survey System (FRSS), Department of Fisheries, Bangladesh. 2017; 33: 124 p.

24. FRSS. Fisheries Statistical Yearbook of Bangladesh. Fisheries Resources Survey System (FRSS), Department of Fisheries, Bangladesh. 2014; 30: 52 p.

25. FRSS. Yearbook of Fisheries Statistics of Bangladesh. Fisheries Resources Survey System (FRSS), Department of Fisheries, Bangladesh. 2016; 33, 56 p.

26. Hasan M, Shahriar Nazrul KM, Parvej MR, Patwary SA, Borhan Uddin AM. Shark and Shark Products Trade Channel and its Conservation Aspects in Bangladesh. J Fisheries Livest Prod. 2017;5(221):2.

27. Shamsuzzaman MM, Xiangmin X, Ming Y, Tania NJ. Towards Sustainable Development of Coastal Fisheries Resources in Bangladesh: An Analysis of the Legal and Institutional Framework. Turk J Fish Aquat Sci. 2017 Jul 1;17(4):833–41.

28. Liu SY, Chan CL, Lin O, Hu CS, Chen CA. DNA barcoding of shark meats identify species composition and CITES-listed species from the markets in Taiwan. PloS one. 2013 Nov 18;8(11): e79373. doi: 10.1371/journal.pone.0079373 24260209

29. Sembiring A, Pertiwi NP, Mahardini A, Wulandari R, Kurniasih EM, Kuncoro AW, et al. DNA barcoding reveals targeted fisheries for endangered sharks in Indonesia. Fish Res. 2015 Apr 30; 164:130–4.

30. Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PD. DNA barcoding Australia's fish species. Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1847–57. doi: 10.1098/rstb.2005.1716 16214743

31. Wynen L, Larson H, Thorburn D, Peverell S, Morgan D, Field I, et al. Mitochondrial DNA supports the identification of two endangered river sharks (Glyphis glyphis and Glyphis garricki) across northern Australia. Mar Freshw Res. 2009 Jul 14;60(6):554–62.

32. Zhang Z, Schwartz S, Wagner L, Miller W. A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000 Feb 1;7(1–2):203–14. doi: 10.1089/10665270050081478 10890397

33. Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, et al. Phylogeny. fr: robust phylogenetic analysis for the non-specialist. Nucleic acids research. 2008 Apr 19;36(suppl_2): W465–9.

34. Dereeper A, Audic S, Claverie JM, Blanc G. BLAST-EXPLORER helps you building datasets for phylogenetic analysis. BMC evolutionary biology. 2010 Dec;10(1):8.

35. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research. 2004 Mar 1;32(5):1792–7. doi: 10.1093/nar/gkh340 15034147

36. Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular biology and evolution. 2000 Apr 1;17(4):540–52. doi: 10.1093/oxfordjournals.molbev.a026334 10742046

37. Guindon S, Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic biology. 2003 Oct 1;52(5):696–704. doi: 10.1080/10635150390235520 14530136

38. Anisimova M, Gascuel O. Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Systematic biology. 2006 Aug 1;55(4):539–52. doi: 10.1080/10635150600755453 16785212

39. Letunic I, Bork P. Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic acids research. 2016 Apr 19;44(W1): W242–5. doi: 10.1093/nar/gkw290 27095192

40. Hossain MA, Thompson BS, Chowdhury GW, Mohsanin S, Fahad ZH, Koldewey HJ, et al. Sawfish exploitation and status in Bangladesh. Aquat Conserv. 2015 Dec;25(6):781–99.

41. O'Malley MP, Townsend KA, Hilton P, Heinrichs S, Stewart JD. Characterization of the trade in manta and devil ray gill plates in China and South‐east Asia through trader surveys. Aquat Conserv. 2017 Apr;27(2):394–413.

42. Abercrombie DL, Clarke SC, Shivji MS. Global-scale genetic identification of hammerhead sharks: application to assessment of the international fin trade and law enforcement. Conserv Genet. 2005 Sep 1;6(5):775–88.

43. Shivji M, Clarke S, Pank M, Natanson L, Kohler N, Stanhope M. Genetic identification of pelagic shark body parts for conservation and trade monitoring. Conserv Biol. 2002 Aug;16(4):1036–47.

44. Chapman DD, Abercrombie DL, Douady CJ, Pikitch EK, Stanhopen MJ, Shivji MS. A streamlined, bi-organelle, multiplex PCR approach to species identification: Application to global conservation and trade monitoring of the great white shark, Carcharodon carcharias. Conserv Genet. 2003 Jul 1;4(4):415–25.

45. Ward RD, Holmes BH, White WT, Last PR. DNA barcoding Australasian chondrichthyans: results and potential uses in conservation. Mar Freshw Res. 2008 Feb 15;59(1):57–71.

46. Frankham R. Genetics and conservation biology. C R Biol. 2003 Aug 1; 326:22–9.

47. Dudgeon CL, Blower DC, Broderick D, Giles JL, Holmes BJ, Kashiwagi T, et al. A review of the application of molecular genetics for fisheries management and conservation of sharks and rays. J Fish Bio. 2012 Apr;80(5):1789–843.

48. Vannuccini S. Shark utilization, marketing and trade. Food & Agriculture Org; 1999.

49. Compagno LJ. Sharks of the world: an annotated and illustrated catalogue of shark species known to date. 1984.

50. Compagno LJ. Sharks of the world: an annotated and illustrated catalogue of shark species known to date. Food & Agriculture Org.; 2001.

51. Last P, Naylor G, Séret B, White W, de Carvalho M, Stehmann M (eds). Rays of the World. Csiro Publishing. 2016.

52. Fields AT, Abercrombie DL, Eng R, Feldheim K, Chapman DD. A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. PloS one. 2015 Feb 3;10(2): e0114844. doi: 10.1371/journal.pone.0114844 25646789

53. Caballero S, Cardenosa D, Soler G, Hyde J. Application of multiplex PCR approaches for shark molecular identification: feasibility and applications for fisheries management and conservation in the Eastern Tropical Pacific. Mol Ecol Resour. 2012 Mar;12(2):233–7. doi: 10.1111/j.1755-0998.2011.03089.x 22067369

54. Castro JI, Woodley CM, Brudeck RL. A preliminary evaluation of the status of shark species. Fisheries technical paper 380. Food and Agriculture Organization, Rome. 1999.

55. Meusnier I, Singer GA, Landry JF, Hickey DA, Hebert PD, Hajibabaei M. A universal DNA mini-barcode for biodiversity analysis. BMC genomics. 2008 Dec;9(1):214.

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