Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

Autoři: Ji Hong Kim aff001;  Chae Hwan Cho aff001;  Myung Yi Ryu aff001;  Jong-Gil Kim aff002;  Sei-Jung Lee aff001;  Tae Jung Park aff002;  Jong Pil Park aff001
Působiště autorů: Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan, Republic of Korea aff001;  Department of Chemistry, Institute of Interdisciplinary Convergence Research, Research Institute of Halal Industrialization Technology, Chung-Ang University, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222144


Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-substituted synthetic peptides was rationally designed, chemically synthesized and covalently immobilized to a gold sensor surface. The sensor performance was monitored via square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides specific for NS1 were chosen according to the dynamic current decrease in SWV experiments. Using circular dichroism, the molar ellipticity of peptides (DGV BP1–BP5) was determined, indicating that they had a mostly similar in random coil structure, not totally identical. Using SWV, DGV BP1 was selected as a promising recognition peptide and limit of detection for NS1 was found to be 1.49 μg/mL by the 3-sigma rule. DGV BP1 showed good specificity and stability for NS1, with low signal interference. The validation of the sensor to detect NS1 proteins was confirmed with four dengue virus culture broth (from serotype 1 to 4) as proof-of-concept. The detection performance of our sensor incorporating DGV BP1 peptides showed a statistically significant difference. These results indicate that this strategy can potentially be used to detect the dengue virus antigen, NS1, and to diagnosis dengue fever within a miniaturized portable device in point-of-care testing.

Klíčová slova:

Dengue virus – Electrochemistry – Sensory systems – Synthetic peptides – Gold – Dengue fever – Biosensors – Square waves


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