Developing cookies formulated with goat cream enriched with conjugated linoleic acid

Autoři: Ana C. S. Costa aff001;  Diego E. Pereira aff001;  Caio M. Veríssimo aff001;  Marcos A. D. Bomfim aff003;  Rita C. R. E. Queiroga aff001;  Marta S. Madruga aff001;  Susana Alves aff005;  Rui J. B. Bessa aff005;  Maria E. G. Oliveira aff001;  Juliana K. B. Soares aff001
Působiště autorů: Food Science and Technology Program, Federal University of Paraíba, João Pessoa, Brazil aff001;  Department of Rural Technology, Federal Rural University of Pernambuco, Recife, Brazil aff002;  Embrapa - Goats and Sheep, Sobral, Brazil aff003;  Bromatology Laboratory, Department of Nutrition, Federal University of Paraíba, João Pessoa, Brazil aff004;  Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal aff005;  Department of Nutrition, Federal University of Campina Grande, Cuité, Brazil aff006
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0212534


Goat fat is one of the best sources of conjugated linoleic acid (CLA), a fatty acid which has health benefits. However, though CLA is generated in ruminants, CLA consumption is limited to meats and milk products. This study aimed to replace vegetable fat with goat milk fat enriched with CLA. From differing fat sources, four cookie recipes were developed: CVF–vegetable fat cookies; CB–butter cookies; CG–goat milk fat cookies without CLA; CGCLA–goat milk fat cookies with CLA. The cookies were evaluated using physical (color and texture), physical-chemical parameters (lipids, proteins, total sugars, fiber, ash, moisture and Aw), consumer testing (n = 123), and lipid profiles. The CGCLA presented higher values in the color parameters. The highest and the lowest scores obtained for hardness were respectively 5.54 (CB) and 2.21 (CVF). Lipids and total sugars varied inversely; the highest percentages of lipids were in the CVF and CG samples which obtained lower total sugar content. There were no differences in acceptance or preference for the four formulations. The goat cream formulations (CG and CGCLA) were as well accepted as the CFV formulation. For lipid profiles, CFV presented the highest percentage of trans-fatty acids (TFA) at 16.76%. CGCLA presented 70% more CLA than either CB or CG, certifying that CGCLA presents CLA in relevant quantities, even after cooking. The CGCLA presented higher levels of CLA, and in this study it was verified that goat milk cream enriched with CLA can be used in producing cookies, adding functional and nutritional properties, and offering another alternative(s) to produce food from goat milk fat.

Klíčová slova:

Fats – Fatty acids – Goats – Lipids – Milk – principal component analysis – Statistical data – Vegetables


1. Mohamed H, El-Salam ABD, El-Shibiny S. Conjugated linoleic acid and vaccenic acid contents in cheeses: An overview from the literature. J. Food Compos. Anal. 2014; 33: 117–126.

2. Koba K, Yanagita T. Health benefits of conjugated linoleic acid (CLA). Obes Res Clin Pract. 2014; 8:525–532.

3. Park Y, Pariza M, The bioactivities and potential mechanisms of action of the conjugated linoleic acid. Food Sci Biotechnol. 2009; 18: 586–593.

4. Lopez-Aliaga I, Alferez MJM, Nestares MT, Ros PB, Barrionuevo M, Campos MS. Goat milk feeding causes an increase in biliary secretion of cholesterol and a decrease in plasma cholesterol levels in rats. J. Dairy Sci. 2005; 88: 1024–1030. doi: 10.3168/jds.S0022-0302(05)72770-3 15738237

5. Soares JKB, Melo APR, Medeiros MAC, Queiroga RCRE, Bomfim MAD, Santiago ECA et al. Anxiety behavior is reduced, and physical growth is improved in theprogeny of rat dams that consumed lipids from goat milk: An elevatedplus maze analysis. Neurosci. Lett. 2013;. 552: 25–29. doi: 10.1016/j.neulet.2013.07.028 23916660

6. Assis POA, Guerra JCB, Araujo DFS, Junior RFA, Machado TADG, Araujo AA, et al. Intestinal anti-inflammatory activity of goat milk and goat yoghurt in the acetic acid model of rat colitis. Int Dairy J. 2016; 56: 45–54.

7. Costa N, Cruz R, Graça P, Breda J, Casal S. Trans fatty acids in the Portuguese food market. Food Control. 2016; 64: 128–134. doi: 10.1016/j.foodcont.2015.12.010 27274619

8. Tarancón P, Fiszman SM, Salvador A, Tárrega A. Formulating biscuit with healthier fats. Consumer profiling of textural and flavor sensations during consumption. Food Res. Int. 2013; 53 (1): 134–140.

9. Pareyt B, Talhaoui F, Kerckhofs G, Brijs K, Goesaert H, et al. The role of sugar and fat in sugar-snap cookies: Structural and textural properties. J Food Eng. 2009; 90 (3) 400–408.

10. Gökmen V, Serpen A, Açar OÇ, Morales FJ. Significance of furosine as eat-induced marker in cookies. J Cereal Sci. 2008; 48 (3): 843–847.

11. Barbosa MQ, Queiroga RCRE, Bertozzo CCMS, Araújo DFS, Oliveira LIG, Silva JYP et al. Effect of diets with goat milk fat supplemented with exercise on anxiety and oxidative stress in the brains of adult rats. Food Functional. 2018; 9: 2891–2901.

12. Sukhija PS, Palmquist DL. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. J Agric Food Chem. 1988; v: 36 -1202–1206.

13. Palmquist DL, Jenkins TC. Challenges with fats and fatty acid methods. J Anim Sci. 2003; 12: 3250–3254.

14. Commission International de L’eclairage (CIE): “Recommendations on Uniform Color Spaces, Color Difference Equations, Psychometric Color Terms”. Supplement No. 2 to CIE Publication No. 15, Colorimetry, Bureau Central de la CIE, Paris, 1978.

15. Association of Official Agricultural Chemists. Official methods of analysis of Association of Official Agricultural Chemists. 19th ed., Ass. Off. Analytical. Chem., Washington, USA. 2012.

16. Folch J, Lees M, Stanley GHS. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957; 226: 497–509. 13428781

17. Henneberg W, Stohmann F. Beiträge zur begründung einer rationallen Fütterung der Wiederkäuer. Praktisch-landwirthschaftliche und chemisch-physiologische Untersuchungen, für Landwirthe und Physiologen. Ueber die Ausnutzung der Futterstoffe durch das volljährige Rind und über Fleischbildung im Körper desselben C. A. Schwetschke und Sohn, Braunschweig, Berlin, Germany, 1864.

18. Dutcosky SD. Análise sensorial de alimentos. Curitiba: Champagnat, 4 ed; 2013.

19. Sigmastat (programa de computador). Versão 3.1. 2009; Point Richmond (Califórnia): Comercial.

20. Lara E, Cortes P, Briones V, Perez M. Structural and physical modifications of corn biscuits during baking process. LWT. 2011; 44: 622–630.

21. Marques GA, Brilhante JFB, Silva DA, Silva EMN. Whey protein as a substitute for wheat in the development of no added sugar cookies. LWT. 2016; 67: 118–126.

22. Laguna L, Salvador A, Sanz T, Fiszman SM. Performance of a resistant starch rich ingredient in the baking and eating quality of short-dough biscuits. LWT. 2011; 44: 737–746.

23. Sharma P, Gujral HS. Extrusion of hulled barley affecting b-glucan and properties of extrudates. Food Bioprocess Tech. 2013; 6: 1374–1389.

24. Bick MA, Fogaça AO. Cookies with different concentrations of quinoa flour in partial replacement of wheat flour. Braz. J. Food Technol. v. 17, n. 2, p. 121–129, 2014.

25. Peleg M, Normand MD. Symmetrized dot-patterns (SDP) of irregular compressive stress strain relationship. J. Texture Stud. 1992; 23: 427–438.

26. Umesha SS, Sai MR, Indiramma AR, Akshitha S, Naidu KA. Enrichment of biscuits with microencapsulated omega-3 fatty acid (Alpha-linolenic acid) rich Garden cress (Lepidium sativum) seed oil: Physical, sensory and storage quality characteristics of biscuits. LWT. 2015; 62: 654–661.

27. Bertolin TE, Centenaro A, Giacomelli B, Reinehr C, Gutkoski LC. Elaboration of biscuits with oatmeal and fat palm with added L-leucine and calcium for sarcopenia. LWT. 2013; 33: (2) 345–354.

28. Ripolles D, Parron JA, Calvo M, Perez MD, Fitzgerald RJ, Sanchez L. Antioxidant activity of co-products from milk fat processing and their enzymatic hydrolysates obtained with different proteolytic preparations. Int Dairy J. 2016; 60: 70–77.

29. Chung H, Cho A, Lim S. Utilization of germinated and heat-moisture treated brown rices in sugar-snap cookies. LWT. 2014; 57: 260–266.

30. Arimi JM, Duggan E, O’sullivan M, Lyng JG, O’riordan ED. Effect of water activity on the crispiness of a biscuit (Crackerbread): Mechanical and acoustic evaluation. Food Res. Int. 2010; 43:1650–1655.

31. Laguna L, Primo-martín C, Varela P, Salvador A, Sanz T. HPMC and inulin as fat replacers in biscuits: Sensory and instrumental evaluation. LWT. 2014; 56: 494–501.

32. Biguzzi C, Schlich P, Lange C. The impact of sugar and fat reduction on perception and liking of biscuits. Food Qual Prefer. 2014; 35: 41–47.

33. Drewnowski A, Nordensten K, Dwyer J. Replacing sugar and fat in cookies: Impact on product quality and preference. Food Qual Prefer. 1998; 9: 13–20.

34. Santos LAT, Cruz R, Casal S. Trans fatty acids in commercial cookies and biscuits: An update of Portuguese Market. Food Control. 2015; 47:141–146.

35. Verruck S, Dantas A, Prudencio ES. Functionality of the components from goat’s milk, recent advances for functional dairy products development and its implications on human health. J. of Funct. Food. v. 52, 243–257, 2019.

36. Gomes LC, Alcalde CR, Santos GT, Feihrmann AC, Molina BSL, Grande PA et al. Concentrate with calcium salts of fatty acids increases the concentration of polyunsaturated fatty acids in milk produced by dairy goats. Small Rumin. Res. 2015; 124: 81–88.

37. Ruiz-Nunez BDA, Dijck-Brouwer J, Muskiet FAJ. The relation of saturated fatty acids with low-grade inflammation and cardiovascular disease. J. Nutri. Biochem. 2016; 36: 1–20.

38. Gómez-Cortés P, Juárez M, Fuente MA. Milk fatty acids and potential health benefits: An updated vision. Trends Food Sci Technol. 2018; 81: 1–9.

39. Ganguly R, Pierce GN. The toxicity of dietary trans fats. Food Chem Toxicol. 2015; 78: 170–176. doi: 10.1016/j.fct.2015.02.004 25684416

40. Ganguly R, Lavallee R, Maddaford TG, Devaney B, Bassett CMC, et al. Ruminant and industrial trans-fatty acid uptake in the heart. J. Nutriti. Biochem.2016; 31: 60–66.

41. Park Y. Conjugated linoleic acid (CLA): Good or bad trans fat?. J. Food Composit Anal. 2009; 22: 4–12.

42. Mele MC, Cannelli G, Carta G, Cordeddu L, Melis MP, Murro E et al. Metabolism of c9,t11-conjugated linoleic acid (CLA) in humans. Prostaglandins Leukot. Essent. Fatty Acids. 2013; 89: 115–119. doi: 10.1016/j.plefa.2013.05.005 23809328

43. Razzaghia A, Naseriana AA, Valizadeha R, Ebrahimia SH.; Khorramia B, Malekkhahia M et al. Pomegranate seed pulp, pistachio hulls, and tomato pomace as replacement of wheat bran increased milk conjugated linoleic acid concentrations without adverse effects on ruminal fermentation and performance of Saanen dairy goats. Anim Feed Sci Technol. 2015; 210: 46–55.

44. Tran NM, Burdejová P, Ospienko M, Härdle WK. Principal component analysis in an asymmetric norm. Journal Multivariate Anal. 2019; 171: 1–21.

45. Piclin N, Pintore M, Lanza CM, Scacco A, Guccione S, Giurato L et al. Sensory analysis of red wines: discrimination by adaptive fuzzy partition. J Sens Stud. 2008; 23: 58–569.

46. Nurgel C, Pickering GJ, Inglis DL. Sensory and chemical characteristics of Canadian ice wines. J Sci Agric. 2004; 84: 1675–1684.

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