Blast vacuolization in AML patients indicates adverse-risk AML and is associated with impaired survival after intensive induction chemotherapy


Autoři: Olivier Ballo aff001;  Jan Stratmann aff001;  Hubert Serve aff001;  Björn Steffen aff001;  Fabian Finkelmeier aff003;  Christian Brandts aff001
Působiště autorů: Department of Medicine, Hematology/Oncology, Goethe University, Theodor-Stern-Kai, Frankfurt/Main, Germany aff001;  German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany aff002;  Department of Medicine, Gastroenterology, Hepatology and Endocrinology, Goethe University, Theodor-Stern-Kai, Frankfurt/Main, Germany aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223013

Souhrn

Introduction

Vacuolization is a frequently found morphological feature in acute myeloid leukemia (AML) blasts. Subcellular origin and biological function as well as prognostic impact are currently unknown. The aim of this study was to evaluate whether vacuolization correlates with clinically relevant AML features.

Materials & methods

Bone marrow smears of patients diagnosed with AML at the University Hospital Frankfurt between January 2011 and August 2013 were analyzed for blast vacuolization and correlated with clinically relevant AML features. Patients undergoing standard induction chemotherapy were further analyzed for molecular and cytogenetic features as well as treatment response and survival.

Results

14 of 100 patients diagnosed with AML receiving standard induction chemotherapy had evidence of blast vacuolization. Positivity for vacuolization correlated with a CD15 positive immunophenotype and with a higher incidence of high-risk AML according to the European LeukemiaNet risk stratification. AML patients with blast vacuolization had a poor blast clearance after standard induction chemotherapy and poor survival.

Discussion

In conclusion, our findings demonstrate that vacuolization can easily be determined in myeloid leukemia blasts and may be a useful biomarker to predict AML risk groups as well as early treatment response rates and survival.

Klíčová slova:

Bone marrow – Cancer chemotherapy – Deletion mutation – Chemotherapy – Vacuoles – Acute myeloid leukemia – Karyotypes – Cytogenetics


Zdroje

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PLOS One


2019 Číslo 9

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