Assessment of glucose-6-phosphate dehydrogenase activity using CareStart G6PD rapid diagnostic test and associated genetic variants in Plasmodium vivax malaria endemic setting in Mauritania


Autoři: Oum kelthoum Mamadou Djigo aff001;  Mohamed Abdallahi Bollahi aff002;  Moina Hasni Ebou aff001;  Mohamed Salem Ould Ahmedou Salem aff001;  Rachida Tahar aff003;  Hervé Bogreau aff004;  Leonardo Basco aff005;  Ali Ould Mohamed Salem Boukhary aff001
Působiště autorů: Unité de recherche Génomes et Milieux, Faculté des Sciences et Techniques, Université de Nouakchott Al-Aasriya, Nouveau Campus Universitaire, Nouakchott, Mauritania aff001;  Centre National de Transfusion Sanguine, Ministère de la Santé, Nouakchott, Mauritania aff002;  UMR 216 MERIT, IRD, Faculté de Pharmacie, Univ. Paris Descartes, Paris, France aff003;  Unité de Parasitologie et d’Entomologie, Institut de Recherche Biomédicale des Armées, IHU-Méditerranée Infection, Marseille, France aff004;  Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France aff005;  IHU-Méditerranée Infection, Marseille, France aff006;  Centre National de Référence du Paludisme, Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Marseille, France aff007
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0220977

Souhrn

Background

Primaquine is recommended by the World Health Organization (WHO) for radical treatment of Plasmodium vivax malaria. This drug is known to provoke acute hemolytic anemia in individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency. Due to lack of data on G6PD deficiency, the use of primaquine has been limited in Africa. In the present study, G6PD deficiency was investigated in blood donors of various ethnic groups living in Nouakchott, a P. vivax endemic area in Mauritania.

Methodology/Principal findings

Venous blood samples from 443 healthy blood donors recruited at the National Transfusion Center in Nouakchott were screened for G6PD activity using the CareStart G6PD deficiency rapid diagnostic test. G6PD allelic variants were investigated using DiaPlexC G6PD genotyping kit that detects African (A-) and Mediterranean (B-) variants. Overall, 50 of 443 (11.3%) individuals (49 [11.8%] men and 1 [3.7%] woman) were phenotypically deficient. Amongst men, Black Africans had the highest prevalence of G6PD deficiency (15 of 100 [15%]) and White Moors the lowest (10 of 168, [5.9%]). The most commonly observed G6PD allelic variants among 44 tested G6PD-deficient men were the African variant A- (202A/376G) in 14 (31.8%), the Mediterranean variant B- (563T) in 13 (29.5%), and the Betica-Selma A- (376G/968C) allelic variant in 6 (13.6%). The Santamaria A- variant (376G/542T) and A variant (376G) were observed in only one and two individuals, respectively. None of the expected variants was observed in 8 (18.2%) of the tested phenotypically G6PD-deficient men.

Conclusion

This is the first published data on G6PD deficiency in Mauritanians. The prevalence of phenotypic G6PD deficiency was relatively high (11.3%). It was mostly associated with either African or Mediterranean variants, in agreement with diverse Arab and Black African origins of the Mauritanian population.

Klíčová slova:

Medicine and health sciences – Hematology – Anemia – Hemolytic anemia – Glucose-6-phosphate dehydrogenase deficiency – Pharmacology – Drugs – Primaquine – Parasitic diseases – Malaria – Tropical diseases – Malaria – Health care – Blood donors – People and places – Population groupings – Ethnicities – African people – Geographical locations – Africa – Mauritania – Biology and life sciences – Parasitology – Parasite groups – Apicomplexa – Plasmodium – Genetics – Mutation – Point mutation


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