Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations

Autoři: Rola M. Labib aff001;  Iriny M. Ayoub aff001;  Haidy E. Michel aff002;  Mina Mehanny aff003;  Verena Kamil aff005;  Meryl Hany aff005;  Mirette Magdy aff005;  Aya Moataz aff005;  Boula Maged aff005;  Ahmed Mohamed aff005
Působiště autorů: Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt aff001;  Department of Pharmcology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt aff002;  Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt aff003;  Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Center for Infection Research (HZI), Saarland University, Saarbrücken, Germany aff004;  Drug Design Program, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt aff005
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0219561


The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo. Essential oils of M. alternifolia and R. officinalis were analyzed using GC/MS. Essential oil-loaded chitosan topical preparations were formulated. Wound healing potential was evaluated in vivo using an excision wound model in rats. GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively. Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group. Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles. The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein. Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.

Klíčová slova:

Biology and life sciences – Biochemistry – Lipids – Oils – Antioxidants – Physiology – Physiological processes – Tissue repair – Wound healing – Nutrition – Diet – Beverages – Tea – Anatomy – Biological tissue – Connective tissue – Granulation tissue – Integumentary system – Hair – Skin – Hair follicles – Medicine and health sciences – Immunology – Immune response – Inflammation – Diagnostic medicine – Signs and symptoms – Pathology and laboratory medicine


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