A plant biostimulant made from the marine brown algae Ascophyllum nodosum and chitosan reduce Fusarium head blight and mycotoxin contamination in wheat


Autoři: L. R. Gunupuru aff001;  J. S. Patel aff001;  M. W. Sumarah aff002;  J. B. Renaud aff002;  E. G. Mantin aff001;  B. Prithiviraj aff001
Působiště autorů: Department of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, Canada aff001;  Agriculture and Agri-Food Canada, London, Ontario, Canada aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0220562

Souhrn

Fusarium head blight (FHB) caused by Fusarium graminearum is a disease that results in yield loss and mycotoxin contamination in wheat globally. This study assessed the effect of a plant biostimulant prepared from a brown macroalga Ascophyllum nodosum (Liquid Seaweed Extract; LSE) alone and in combination with chitosan in controlling Fusarium. Wheat seedlings drenched with LSE and chitosan in combination showed reduced severity of F. graminearum infection on leaves as evidenced by a significant reduction in necrotic area and fewer number of conidia produced in the necrotic area. Gene expression studies showed that the combination of LSE and chitosan amplified the response of pathogenesis-related genes (TaPR1.1, TaPR2, TaPR3, TaGlu2) in wheat seedlings infected with Fusarium spores above that observed for the individual treatments. The combination treatments were more effective in enhancing the activity of various defense related enzymes such as peroxidase and polyphenol oxidase. FHB studies on adult plants showed a reduction of bleached spikes in wheat heads treated with the combination of LSE and chitosan. Mycotoxin content appeared to be correlated with FHB severity. Combination treatments of LSE and chitosan reduced the levels of mycotoxins deoxynivalenol and sambucinol in wheat grains. Systemic disease resistance appears to be induced by LSE and chitosan in response to F. graminearum in wheat by inducing defense genes and enzymes.

Klíčová slova:

Biology and life sciences – Organisms – Eukaryota – Plants – Grasses – Wheat – Seedlings – Microbiology – Medical microbiology – Microbial pathogens – Fungal pathogens – Fusarium – Microbial control – Mycology – Plant science – Plant anatomy – Leaves – Plant physiology – Plant defenses – Plant pathology – Plant pathogens – Plant fungal pathogens – Medicine and health sciences – Pathology and laboratory medicine – Pathogens – Pharmacology – Antimicrobial resistance


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