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Correction: Metagenomic sequencing suggests a diversity of RNA interference-like responses to viruses across multicellular eukaryotes


Authors: Fergal M. Waldron;  Graham N. Stone;  Darren J. Obbard
Published in the journal: Correction: Metagenomic sequencing suggests a diversity of RNA interference-like responses to viruses across multicellular eukaryotes. PLoS Genet 16(3): e32767. doi:10.1371/journal.pgen.1008679
Category: Correction
doi: https://doi.org/10.1371/journal.pgen.1008679

There are two errors in the article [1] that the authors would like to bring to the readers’ attention.

Firstly, this article reports that miRNAs are likely to be 3' 2'-0-methylated in the cnidarian, Actinia equina. The authors fail to note that this has previously been shown for Nematostella miRNAs in Supplementary table 1 in Grimson et al [2].

In the Results section under the subheading “New virus-like sequences identified by metagenomic sequencing”, there is an error in the penultimate sentence.

Specifically, the authors state with reference to Barns Ness dog whelk orthomyxo-like virus 1, that “the PB2 polymerase subunit falls between Infectious Salmon Anaemia virus and the Influenza/Thogoto virus clade”. This is incorrect, as PB2 should read PB1.

The correct sentence is: These include the Caledonia dog whelk rhabdo-like virus 2 sequence, which is represented by a nucleoprotein that falls between the Rabies/Lyssaviruses and other rhabdoviruses, and Barns Ness dog whelk orthomyxo-like virus 1—for which the PB1 polymerase subunit falls between Infectious Salmon Anaemia virus and the Influenza/Thogoto virus clade (Fig 2; the PA polymerase subunit shows similarity to the Thogoto viruses, but not other Orthomyxoviruses).

The authors do not report, or analyse, the PB2 subunit of Barns Ness dog whelk orthomyxo-like virus 1 in this paper. The sequences for the Barns Ness dog whelk orthomyxo-like virus 1 PB1 and PA subunits are available through GenBank (accession numbers MF190044, MF190045).


Zdroje

1. Waldron FM, Stone GN, Obbard DJ (2018) Metagenomic sequencing suggests a diversity of RNA interference-like responses to viruses across multicellular eukaryotes. PLoS Genet 14(7): e1007533. https://doi.org/10.1371/journal.pgen.1007533 30059538

2. Grimson A, Srivastava M, Fahey B, Woodcroft BJ, Chiang HR, et al. (2008) Early origins and evolution of microRNAs and Piwi-interacting RNAs in animals. Nature 455: 1193–1197. doi: 10.1038/nature07415 18830242


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